Intracellular mechanisms underlying prostaglandin F2a-stimulated phasic myometrial contractions

Phillippe, Mark, Trevania Saunders, and Andrew Basa. Intracellular mechanisms underlying prostaglandin F2a-stimulated phasic myometrial contractions. Am. J. Physiol. 273 (Endocrinol. Metab. 36): E665–E673, 1997.—These studies sought to test the hypothesis that prostaglandin F2a (PGF2a)-stimulated phasic myometrial contractions are characterized by the activation of the phosphatidylinositolsignaling pathway resulting in the generation of cytosolic calcium oscillations. For the experiments described in this report rat myometrial tissue was used, after the tissue was loaded with fura 2, to perform cytosolic calcium imaging studies and to perform computer-digitalized in vitro isometric contraction studies. Consistent with the above hypothesis, the cytosolic calcium-imaging studies demonstrated PGF2astimulated cytosolic calcium oscillations occurring simultaneously with phasic contractions. The in vitro isometric contraction studies confirmed that previously reported inhibitors of the phosphatidylinositol-signaling pathway and cytosolic calcium oscillation mechanisms resulted in significant inhibition of PGF2a-stimulated phasic myometrial contractions. In summary, these studies have provided substantial support for the hypothesis that PGF2a-stimulated phasic myometrial contractions are generated by intracellular signaling mechanisms involving activation of the phosphatidylinositol-signaling pathway and the production of cytosolic calcium oscillation-like phenomena.